Microbial Keystone Expression System

Cytovance’s® Keystone expression system is an E. coli expression platform using a toolbox approach to gene design, host construction, and screening evaluation. Partnering DNA2.0’s GeneGPSTM codon optimization with Cytovance’s® toolbox creates an IP-free and robust method for developing an integrated manufacturing process that addresses challenges unique to each molecule and circumvents pitfalls that can arise in manufacturing.

Keystone Expression SystemTM Features: 

  • E. coli platform designed by Cytovance® personnel based on lessons learned from 10 years of contract bio-manufacturing experience.

  • Quality-by-design approach from gene construction to GMP manufacturing.

  • Proprietary vectors, E. coli hosts, and platform fermentation processes are designed to fit each other for efficient and robust scale-up.

  • Efficient primary screen to evaluate all best systems at once for expressing your product in E. coli (cytoplasmic/periplasmic; fast/slow induction kinetics; soluble/inclusion bodies)

  • Platform fermentation that routinely yields high cell density and high titer in the first run. Fermentation process development focuses on robustness.

  • Efficient work-flow enables early MCB initiation with growth and titer data from a fed-batch process that is scaleable and manufacturable.

Cytovance® staff also has experience working with other microbial systems including Saccharomyces, Pichia pastoris, and Baculovirus.